Transcriptional RNA Universal Multi-Purpose GatE PlaTform
TRUMPET is a biocomputing platform. Operation on Boolean logic gates are performed using protein enzymes operating on DNA templates.
The final readout of the state of the gate is done by transcribing the final template to RNA, with fluorescent RNA aptamer providing 0 (no fluorescence) or 1 (fluorescence detected) signal.
TRUMPET can implement all universal Boolean logic gates.
The most extensive testing has so far been done on NAND gates. TRUMPET will support NOR, AND, OR and NOT gates.
The TRUMPET system can be used in high throughput mode, using pipetting robots.
Multiplexing different gates, and full orthogonality of gates, enables scaling the system up to build complex circuits.